01 / yeast · culture
DIY Yeast Biolab
Some time ago, I set out to knock out yeast PDE2 as a first step in playing around with signalling proteins... In this short piece I wanted to give a brief lab tour...
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All things bio for a solarpunk tomorrow
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Biohacker, passionate about gene editing for medical and ecological purposes. I have been in the longevity space since 2024. Ever since, I’ve been reprogramming, amplifying, purifying… sometimes in my own hobby lab.
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02 / primers · molecular bio
Atypical Primers for Tricky Yeast Genomic Regions
Firstly, why break the rules? Look at the PDE2 yeast genomic region (from the SGD). Look at the parts before and after the PDE2 coding region. That’s a lot of As and Ts...
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03 / purification · biochemistry
Household reagents DNA purification
Normally, in ethanol purification, you need specific salts and a fancy centrifuge for specific speeds. I could get my hands on ethanol. I had a mini centrifuge with one speed, and table salt. Good enough?
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